Amplified ribosomal dna restriction analysis pdf
Physicochemical Analysis After collection in the on-site laboratory, sludge samples were analyzed using conventional techniques as follows: sludge and air temperature, sludge volume index (SVI) [22],
Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. 8 Pages. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene . Author. Denis Roy. Download with Google Download with Facebook or …
ARDRA stands for “Amplified Ribosomal DNA Restriction Analysis”. Q: A: How to abbreviate “Amplified Ribosomal DNA Restriction Analysis”? “Amplified Ribosomal DNA Restriction Analysis” can be abbreviated as ARDRA. Q: A: What is the meaning of ARDRA abbreviation? The meaning of ARDRA abbreviation is “Amplified Ribosomal DNA Restriction Analysis”. Q: A: What …
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
of these methods are based on PCR and restriction site analysis. Some of these methods are still not economic in use and Some of these methods are still not economic in use and require huge set-up cost.
Read “Amplified ribosomal DNA restriction analysis in the differentiation of related species of mycobacteria, Journal Of Microbiological Methods” on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Based on Amplified Ribosomal DNA Restriction Analysis (ARDRA) DIANA ELIZABETH WATURANGI ∗∗∗ , IVANA FRANCISCA, CINDY OKTAVIA SUSANTO Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia,
Use of Amplified Ribosomal DNA Restriction Analysis for

Evolutionary Relationships of Lentinus to the Polyporaceae
Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1500 bp fragments of rDNA amplified by polymerase chain reaction did not discriminate the genomic variations among the eight strains of T. equigenitalis.
enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species ofthe genus Mycobacterium were enzymatically amplified.
Practical identification of human originated Lactobacillus species by amplified ribosomal DNA restriction analysis (ARDRA) for probiotic use
In this context, the potential of amplified ribosomal DNA restriction analysis (ARDRA) for the characterization and differentiation of Azotobacteraceae was evaluated, and the more appropriate tool for the elaboration of data was assessed.
Evolutionary Relationships of Lentinus to the Polyporaceae: Evidence from Restriction Analysis of Enzymatically Amplified Ribosomal DNA David S. Hibbett; Rytas Vilgalys
A total of 52 isolates of Pasteurella pneumotropica obtained from rodents were examined for their genetic heterogeneity. On the basis of DNA restriction analysis, including amplified 16S ribosomal DNA restriction analysis (ARDRA) and pulsed-field gel electrophoresis (PFGE), differences were identified among the isolates.
It is important to determine the minimum number of restriction enzymes for examining the phylogenetic affiliation of the many isolates from environments using RFLP analysis of PCR amplified interspacer rDNA (17). Sneath and Sokal. Dendrogram obtained by unweighed pair group method using arithmetic averages (UPGMA. this strain was grouped with its original group I strains at 55% similarity. PCR
Use of amplified ribosomal DNA restriction analysis for identification of Ralstonia and Pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis.
An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus
PDF Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA

Olaf Schmidt and Ute Moreth, Identification of the Dry Rot Fungus, Serpula lacrymans, and the Wild Merulius, S. himantioides, by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Holzforschung, 53, 2, (1999).
This corrects the article “Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.” on page 2063. Full text Get a printable copy (PDF file) of the complete article (200K), or click on a page image below to browse page by page.
amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samplesof
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
Seven Euplotes spp. were investigated using amplified ribosomal DNA restriction analysis (RFLP, riboprinting). This involved the use of the polymerase chain reaction (PCR) to amplify a segment of the ciliate genome, followed by restriction digestion to yield restriction fragment length polymorphisms among the seven euplotids.
Amplified ribosomal DNA restriction analysis (ARDRA) of new isolated strains of bifidobacteria from newborn babies microbiota. microbial flora, microorganisms, neonates, pH, probiotics, ribosomal DNA, strains, tolerance Identifier(s) : bacterium, gall, gut flora
ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by …
discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. The internally transcribed spacer sequences situated between the 16S and the 23 S rRNA were amplified by polymerase chain reaction and yielded amplicons of about 540 bp. Direct use of cells for polymerase chain reaction seemed to

ABSTRACT. Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting.
Comparison of 16S amplified ribosomal DNA restriction analysis (ARDRA) patterns of fourteen reference Lactobacillus species The in silico patterns obtained from the almost complete 16S-rRNA analysis of 14 Lactobacillus species were compared with experimental patterns of 14 reference Lactobacillus species.
RIBOSOMAL DNA RESTRICTION ENZYME ANALYSIS (ARDRA) N.H. Todorova1, G ARDRA (Amplified Ribosomal DNA Restriction enzyme Analysis) fingerprint method is chosen for assessment of dominant members of bacterial communities in root zone sediment of pristine Zostera meadows as well as in affected ones, as it allows the simultaneous analysis of multiple samples, …
Bipolaris sorokiniana (Sacc. In Sorok) Shoem. is a phytopathogenic fungus that affects rye, barley, oat, wheat, and other gramineous plants. The pathogen has a worldwide distribution, but is particularly aggressive under conditions of high humidity and temperature.
The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production
564 (Yamamoto & Harayama, 1996) and recA-RFLP (Jawad et al., 1998). Among these, amplified ribosomal DNA restriction analysis (ARDRA) was reported to be the useful
Molecular Typing of Pasteurella pneumotropica Isolated
This study showed that a polymerase chain reaction named ARDRA (Amplified 16S Ribosomal DNA Restriction Analysis) method could prove to be an invaluable insight for molecular identification of the A. paragallinarum, a microorganism implicated in respiratory diseases in commercial birds.
How to Cite this Article: Afaf I. Shehata, et al,. “Molecular Identification Of Probiotics Lactobacillus Strain isolates by: Amplified Ribosomal DNA Restriction Analysis (AR-
Amplified ribosomal DNA restriction analysis (ARDRA) in which the almost complete 16S rRNA gene was used as the target of the PCR assay and randomly and repetitive element-primed PCR were used to characterize 67 strains belonging to the family Alcaligenaceae.
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species.
Amplified Ribosomal DNA Restriction Analysis. Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.
Results demonstrate that the amplified ribosomal DNA restriction analysis (ARDRA) method allows G. vaginalis to be separated into at least 4 homogeneous genotypes, some of which showed a prevalent distribution in certain of the centers the strains were derived from.
Amplified ribosomal DNA restriction analysis (ARDRA) proved to be useful for the classification of bacterial strains at different taxonomic levels and to analyze the genetic variability between bacterial isolates depending on selection of conserved or variable regions in the ribosomal … – natures purest hug me swing instructions Molecular biological methods based on genus-specific PCR, species-specific PCR, and amplified ribosomal DNA restriction analysis (ARDRA) of two PCR amplicons (523 and 914 bp) using six restriction enzymes were used to differentiate among species of Bifidobacterium.
Amplified ribosomal DNA restriction analysis (ARDRA) has an excellent potential for discrimination of organisms at the species level [6,7]. This technique is based on the amplification of the DNA sequence of a 16S rDNA region, followed by the digestion of PCR products with restriction enzymes [ 6 , 7 ].
Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia Kong, B.H. and Hanifah, Y.A. and Yusof, M.Y. and Thong, Kwai Lin (2011) Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia.
Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified …
see more details) were used to identify the differentiation by amplified ribosomal DNA ribosomal dna Subject Category: Chemicals and Chemical Groups see more details restriction analysis (ARDRA). 16S rDNA was amplified by the general primer pair 16S -27 and 16S -1525.
A rapid procedure for the identification of cultured Mycobacterium isolates, based on the combination of enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species of the genus Mycobacterium were enzymatically amplified
Read “Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae : a contribution to the study of these free-living nitrogen-fixing bacteria, Journal Of Microbiological Methods” on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Ribosomal DNA was amplified separately from 3 individuals for each population of Cx. pipiens and analyzed by restriction digestion. Intrapopulation variation is seen, because for each
Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal …
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
fig11379: Amplified Ribosomal DNA Restriction Analysis Using Taq1 (TCGA) Mentions: Figure 2 demonstrates that all the isolates of 1114 bp were specific to bacilli. Taq1 digested the PCR product identically and produced two amplicons with approximate sizes of 420 and 690 bp (Figure 3).
˜2016 152 115 Identifying the Origin of Forensic Soil Evidence Using Amplified Ribosomal DNA Restriction Analysis of its Bacterial Community Venus Naknim1, Wibhu Kutanan2 and …
RFLP analysis of PCR-amplified small subunit ribosomal DNA
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of
2 • Based on 16S ribosomal DNA gene (and possibly other genes) – 16S rDNA libraries and sequencing – Amplified ribosomal DNA restriction analysis (ARDRA)
We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this
ISSN 0564–3783. Öèòîëîãèÿ è ãåíåòèêà. 2014. Ò. 48. ¹ 5 27 Genetic diversity of Azotobacter strains isolated from soils by amplified ribosomal DNA restriction analysis
An Application of Amplified Ribosomal DNA Restriction Analysis in the Changes of Microbial Community Structure of Industrial Waste Water Treatment by M. Shah International Journal of Environmental Bioremediation & Biodegradation .
The phylogenetic relationships of the microsporidian species Microgemma caulleryi, Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA).
The polymerase chain reaction (PCR) and subsequent analysis of amplified rDNA using restriction endonucleases were employed in different studies topecies discrimination in achieve efficient inters medical [7] and food [8] mycology.
electrophoresis of fragments of ribosomal RNA gene (rDNA), generated by PCR, and sub- sequent digestions with restriction enzymes (ARDRA – amplified ribosomal DNA restriction analysis).
Amplified ribosomal DNA restriction analysis (ARDRA) For each isolate, 10 ll of PCR-amplified 16S rDNA was digested for 4 h at 37 C with 5 U of restriction endonuclease CfoI, HinfI, RsaI, DdeI, Sau3AI, AluI, HaeIII, and MspI (Bangalore GeNei, India) according to the manufacturer’s instructions and separated by electrophoresis on 2 % agarose gels in 19 Tris–acetate–EDTA buffer for 2 h at
Restriction analysis of the amplified ribosomal DNA

Identification of Acinetobacter genomic species by
A total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (DNA groups) of the genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA).
Amplified Ribosomal DNA Restriction Analysis as a Tool to

Molecular differentiation of Bifidobacterium species with
Amplified Ribosomal DNA Restriction Analysis Using Taq1
Use of amplified ribosomal DNA restriction analysis for
An Application of Amplified Ribosomal DNA Restriction
– Amplified ribosomal DNA restriction analysis in DeepDyve
Amplified ribosomal DNA restriction analysis as a routine
jurnal RFLP.pdf Gel Electrophoresis Agarose Gel
The Variability in the Fungal Ribosomal DNA (ITS1 ITS2
Identification of Mycobacterium by Amplified Ribosomal DNA
An Application of Amplified Ribosomal DNA Restriction
ISSN 0564–3783. Öèòîëîãèÿ è ãåíåòèêà. 2014. Ò. 48. ¹ 5 27 Genetic diversity of Azotobacter strains isolated from soils by amplified ribosomal DNA restriction analysis
of these methods are based on PCR and restriction site analysis. Some of these methods are still not economic in use and Some of these methods are still not economic in use and require huge set-up cost.
The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production
Molecular biological methods based on genus-specific PCR, species-specific PCR, and amplified ribosomal DNA restriction analysis (ARDRA) of two PCR amplicons (523 and 914 bp) using six restriction enzymes were used to differentiate among species of Bifidobacterium.
Physicochemical Analysis After collection in the on-site laboratory, sludge samples were analyzed using conventional techniques as follows: sludge and air temperature, sludge volume index (SVI) [22],
amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samplesof
Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. 8 Pages. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene . Author. Denis Roy. Download with Google Download with Facebook or …
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
see more details) were used to identify the differentiation by amplified ribosomal DNA ribosomal dna Subject Category: Chemicals and Chemical Groups see more details restriction analysis (ARDRA). 16S rDNA was amplified by the general primer pair 16S -27 and 16S -1525.
Use of amplified ribosomal DNA restriction analysis for
Identification of Acinetobacter genomic species by
This corrects the article “Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.” on page 2063. Full text Get a printable copy (PDF file) of the complete article (200K), or click on a page image below to browse page by page.
discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. The internally transcribed spacer sequences situated between the 16S and the 23 S rRNA were amplified by polymerase chain reaction and yielded amplicons of about 540 bp. Direct use of cells for polymerase chain reaction seemed to
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this
The polymerase chain reaction (PCR) and subsequent analysis of amplified rDNA using restriction endonucleases were employed in different studies topecies discrimination in achieve efficient inters medical [7] and food [8] mycology.
Use of amplified ribosomal DNA restriction analysis for identification of Ralstonia and Pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis.
Identification of Mycobacterium species by using amplified
Evaluation of amplified ribosomal DNA restriction analysis
564 (Yamamoto & Harayama, 1996) and recA-RFLP (Jawad et al., 1998). Among these, amplified ribosomal DNA restriction analysis (ARDRA) was reported to be the useful
Amplified ribosomal DNA restriction analysis (ARDRA) For each isolate, 10 ll of PCR-amplified 16S rDNA was digested for 4 h at 37 C with 5 U of restriction endonuclease CfoI, HinfI, RsaI, DdeI, Sau3AI, AluI, HaeIII, and MspI (Bangalore GeNei, India) according to the manufacturer’s instructions and separated by electrophoresis on 2 % agarose gels in 19 Tris–acetate–EDTA buffer for 2 h at
discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. The internally transcribed spacer sequences situated between the 16S and the 23 S rRNA were amplified by polymerase chain reaction and yielded amplicons of about 540 bp. Direct use of cells for polymerase chain reaction seemed to
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying
A total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (DNA groups) of the genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA).
The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species.
Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1500 bp fragments of rDNA amplified by polymerase chain reaction did not discriminate the genomic variations among the eight strains of T. equigenitalis.
PDF Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA
Physicochemical Analysis After collection in the on-site laboratory, sludge samples were analyzed using conventional techniques as follows: sludge and air temperature, sludge volume index (SVI) [22],
Seven Euplotes spp. were investigated using amplified ribosomal DNA restriction analysis (RFLP, riboprinting). This involved the use of the polymerase chain reaction (PCR) to amplify a segment of the ciliate genome, followed by restriction digestion to yield restriction fragment length polymorphisms among the seven euplotids.
It is important to determine the minimum number of restriction enzymes for examining the phylogenetic affiliation of the many isolates from environments using RFLP analysis of PCR amplified interspacer rDNA (17). Sneath and Sokal. Dendrogram obtained by unweighed pair group method using arithmetic averages (UPGMA. this strain was grouped with its original group I strains at 55% similarity. PCR
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
ABSTRACT. Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting.
Identification of Weissella species by the genus‐specific
Molecular characterization and amplified ribosomal DNA
This study showed that a polymerase chain reaction named ARDRA (Amplified 16S Ribosomal DNA Restriction Analysis) method could prove to be an invaluable insight for molecular identification of the A. paragallinarum, a microorganism implicated in respiratory diseases in commercial birds.
ISSN 0564–3783. Öèòîëîãèÿ è ãåíåòèêà. 2014. Ò. 48. ¹ 5 27 Genetic diversity of Azotobacter strains isolated from soils by amplified ribosomal DNA restriction analysis
Use of amplified ribosomal DNA restriction analysis for identification of Ralstonia and Pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis.
Bipolaris sorokiniana (Sacc. In Sorok) Shoem. is a phytopathogenic fungus that affects rye, barley, oat, wheat, and other gramineous plants. The pathogen has a worldwide distribution, but is particularly aggressive under conditions of high humidity and temperature.
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this
Use of amplified ribosomal DNA restriction analysis for
Amplified ribosomal DNA restriction analysis for the
ISSN 0564–3783. Öèòîëîãèÿ è ãåíåòèêà. 2014. Ò. 48. ¹ 5 27 Genetic diversity of Azotobacter strains isolated from soils by amplified ribosomal DNA restriction analysis
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
The polymerase chain reaction (PCR) and subsequent analysis of amplified rDNA using restriction endonucleases were employed in different studies topecies discrimination in achieve efficient inters medical [7] and food [8] mycology.
Use of amplified ribosomal DNA restriction analysis for identification of Ralstonia and Pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis.
This study showed that a polymerase chain reaction named ARDRA (Amplified 16S Ribosomal DNA Restriction Analysis) method could prove to be an invaluable insight for molecular identification of the A. paragallinarum, a microorganism implicated in respiratory diseases in commercial birds.
Olaf Schmidt and Ute Moreth, Identification of the Dry Rot Fungus, Serpula lacrymans, and the Wild Merulius, S. himantioides, by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Holzforschung, 53, 2, (1999).
ABSTRACT. Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting.
The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species.
fig11379: Amplified Ribosomal DNA Restriction Analysis Using Taq1 (TCGA) Mentions: Figure 2 demonstrates that all the isolates of 1114 bp were specific to bacilli. Taq1 digested the PCR product identically and produced two amplicons with approximate sizes of 420 and 690 bp (Figure 3).
Amplified ribosomal DNA restriction analysis (ARDRA) For each isolate, 10 ll of PCR-amplified 16S rDNA was digested for 4 h at 37 C with 5 U of restriction endonuclease CfoI, HinfI, RsaI, DdeI, Sau3AI, AluI, HaeIII, and MspI (Bangalore GeNei, India) according to the manufacturer’s instructions and separated by electrophoresis on 2 % agarose gels in 19 Tris–acetate–EDTA buffer for 2 h at
Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. 8 Pages. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene . Author. Denis Roy. Download with Google Download with Facebook or …
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying
A rapid procedure for the identification of cultured Mycobacterium isolates, based on the combination of enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species of the genus Mycobacterium were enzymatically amplified
Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified …
˜2016 152 115 Identifying the Origin of Forensic Soil Evidence Using Amplified Ribosomal DNA Restriction Analysis of its Bacterial Community Venus Naknim1, Wibhu Kutanan2 and …
Identification of Weissella species by the genus‐specific
Identification of Mycobacterium Species by Using Amplified
Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. 8 Pages. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene . Author. Denis Roy. Download with Google Download with Facebook or …
A total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (DNA groups) of the genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA).
Molecular biological methods based on genus-specific PCR, species-specific PCR, and amplified ribosomal DNA restriction analysis (ARDRA) of two PCR amplicons (523 and 914 bp) using six restriction enzymes were used to differentiate among species of Bifidobacterium.
Ribosomal DNA was amplified separately from 3 individuals for each population of Cx. pipiens and analyzed by restriction digestion. Intrapopulation variation is seen, because for each
Olaf Schmidt and Ute Moreth, Identification of the Dry Rot Fungus, Serpula lacrymans, and the Wild Merulius, S. himantioides, by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Holzforschung, 53, 2, (1999).
Based on Amplified Ribosomal DNA Restriction Analysis (ARDRA) DIANA ELIZABETH WATURANGI ∗∗∗ , IVANA FRANCISCA, CINDY OKTAVIA SUSANTO Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia,
Bipolaris sorokiniana (Sacc. In Sorok) Shoem. is a phytopathogenic fungus that affects rye, barley, oat, wheat, and other gramineous plants. The pathogen has a worldwide distribution, but is particularly aggressive under conditions of high humidity and temperature.
The polymerase chain reaction (PCR) and subsequent analysis of amplified rDNA using restriction endonucleases were employed in different studies topecies discrimination in achieve efficient inters medical [7] and food [8] mycology.
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
Seven Euplotes spp. were investigated using amplified ribosomal DNA restriction analysis (RFLP, riboprinting). This involved the use of the polymerase chain reaction (PCR) to amplify a segment of the ciliate genome, followed by restriction digestion to yield restriction fragment length polymorphisms among the seven euplotids.
ARDRA stands for “Amplified Ribosomal DNA Restriction Analysis”. Q: A: How to abbreviate “Amplified Ribosomal DNA Restriction Analysis”? “Amplified Ribosomal DNA Restriction Analysis” can be abbreviated as ARDRA. Q: A: What is the meaning of ARDRA abbreviation? The meaning of ARDRA abbreviation is “Amplified Ribosomal DNA Restriction Analysis”. Q: A: What …
enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species ofthe genus Mycobacterium were enzymatically amplified.
Evaluation of amplified ribosomal DNA restriction analysis
Genetic Diversity of Methylotrophic Bacteria from Human
Practical identification of human originated Lactobacillus species by amplified ribosomal DNA restriction analysis (ARDRA) for probiotic use
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
Amplified ribosomal DNA restriction analysis (ARDRA) in which the almost complete 16S rRNA gene was used as the target of the PCR assay and randomly and repetitive element-primed PCR were used to characterize 67 strains belonging to the family Alcaligenaceae.
Amplified ribosomal DNA restriction analysis (ARDRA) proved to be useful for the classification of bacterial strains at different taxonomic levels and to analyze the genetic variability between bacterial isolates depending on selection of conserved or variable regions in the ribosomal …
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
Based on Amplified Ribosomal DNA Restriction Analysis (ARDRA) DIANA ELIZABETH WATURANGI ∗∗∗ , IVANA FRANCISCA, CINDY OKTAVIA SUSANTO Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia,
ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by …
ARDRA stands for “Amplified Ribosomal DNA Restriction Analysis”. Q: A: How to abbreviate “Amplified Ribosomal DNA Restriction Analysis”? “Amplified Ribosomal DNA Restriction Analysis” can be abbreviated as ARDRA. Q: A: What is the meaning of ARDRA abbreviation? The meaning of ARDRA abbreviation is “Amplified Ribosomal DNA Restriction Analysis”. Q: A: What …
The polymerase chain reaction (PCR) and subsequent analysis of amplified rDNA using restriction endonucleases were employed in different studies topecies discrimination in achieve efficient inters medical [7] and food [8] mycology.
A total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (DNA groups) of the genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA).
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
Amplified Ribosomal DNA Restriction Analysis. Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.
Ribosomal DNA was amplified separately from 3 individuals for each population of Cx. pipiens and analyzed by restriction digestion. Intrapopulation variation is seen, because for each
How to Cite this Article: Afaf I. Shehata, et al,. “Molecular Identification Of Probiotics Lactobacillus Strain isolates by: Amplified Ribosomal DNA Restriction Analysis (AR-
Amplified ribosomal DNA restriction analysis of free
Amplified Ribosomal DNA Restriction Analysis
This corrects the article “Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.” on page 2063. Full text Get a printable copy (PDF file) of the complete article (200K), or click on a page image below to browse page by page.
The phylogenetic relationships of the microsporidian species Microgemma caulleryi, Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA).
of these methods are based on PCR and restriction site analysis. Some of these methods are still not economic in use and Some of these methods are still not economic in use and require huge set-up cost.
Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia Kong, B.H. and Hanifah, Y.A. and Yusof, M.Y. and Thong, Kwai Lin (2011) Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia.
A total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (DNA groups) of the genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA).
Amplified Ribosomal DNA Restriction Analysis. Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.
ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by …
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
Seven Euplotes spp. were investigated using amplified ribosomal DNA restriction analysis (RFLP, riboprinting). This involved the use of the polymerase chain reaction (PCR) to amplify a segment of the ciliate genome, followed by restriction digestion to yield restriction fragment length polymorphisms among the seven euplotids.
Amplified ribosomal DNA restriction analysis (ARDRA) has an excellent potential for discrimination of organisms at the species level [6,7]. This technique is based on the amplification of the DNA sequence of a 16S rDNA region, followed by the digestion of PCR products with restriction enzymes [ 6 , 7 ].
˜2016 152 115 Identifying the Origin of Forensic Soil Evidence Using Amplified Ribosomal DNA Restriction Analysis of its Bacterial Community Venus Naknim1, Wibhu Kutanan2 and …
Comparison of 16S amplified ribosomal DNA restriction analysis (ARDRA) patterns of fourteen reference Lactobacillus species The in silico patterns obtained from the almost complete 16S-rRNA analysis of 14 Lactobacillus species were compared with experimental patterns of 14 reference Lactobacillus species.
Amplified ribosomal DNA restriction analysis as a routine
Use of amplified ribosomal DNA restriction analysis for
Olaf Schmidt and Ute Moreth, Identification of the Dry Rot Fungus, Serpula lacrymans, and the Wild Merulius, S. himantioides, by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Holzforschung, 53, 2, (1999).
An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus
We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this
Amplified Ribosomal DNA Restriction Analysis. Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
This study showed that a polymerase chain reaction named ARDRA (Amplified 16S Ribosomal DNA Restriction Analysis) method could prove to be an invaluable insight for molecular identification of the A. paragallinarum, a microorganism implicated in respiratory diseases in commercial birds.
Evolutionary Relationships of Lentinus to the Polyporaceae: Evidence from Restriction Analysis of Enzymatically Amplified Ribosomal DNA David S. Hibbett; Rytas Vilgalys
amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samplesof
In this context, the potential of amplified ribosomal DNA restriction analysis (ARDRA) for the characterization and differentiation of Azotobacteraceae was evaluated, and the more appropriate tool for the elaboration of data was assessed.
Bipolaris sorokiniana (Sacc. In Sorok) Shoem. is a phytopathogenic fungus that affects rye, barley, oat, wheat, and other gramineous plants. The pathogen has a worldwide distribution, but is particularly aggressive under conditions of high humidity and temperature.
Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified …
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
Amplified Ribosomal DNA Restriction Analysis as a Tool to
Read “Amplified ribosomal DNA restriction analysis in the differentiation of related species of mycobacteria, Journal Of Microbiological Methods” on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
An Application of Amplified Ribosomal DNA Restriction Analysis in the Changes of Microbial Community Structure of Industrial Waste Water Treatment by M. Shah International Journal of Environmental Bioremediation & Biodegradation .
Amplified ribosomal DNA restriction analysis (ARDRA) of new isolated strains of bifidobacteria from newborn babies microbiota. microbial flora, microorganisms, neonates, pH, probiotics, ribosomal DNA, strains, tolerance Identifier(s) : bacterium, gall, gut flora
Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia Kong, B.H. and Hanifah, Y.A. and Yusof, M.Y. and Thong, Kwai Lin (2011) Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia.
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
It is important to determine the minimum number of restriction enzymes for examining the phylogenetic affiliation of the many isolates from environments using RFLP analysis of PCR amplified interspacer rDNA (17). Sneath and Sokal. Dendrogram obtained by unweighed pair group method using arithmetic averages (UPGMA. this strain was grouped with its original group I strains at 55% similarity. PCR
Molecular biological methods based on genus-specific PCR, species-specific PCR, and amplified ribosomal DNA restriction analysis (ARDRA) of two PCR amplicons (523 and 914 bp) using six restriction enzymes were used to differentiate among species of Bifidobacterium.
Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of
A rapid procedure for the identification of cultured Mycobacterium isolates, based on the combination of enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species of the genus Mycobacterium were enzymatically amplified
Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal …
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
Ribosomal DNA was amplified separately from 3 individuals for each population of Cx. pipiens and analyzed by restriction digestion. Intrapopulation variation is seen, because for each
ABSTRACT. Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting.
Amplified ribosomal DNA restriction analysis in DeepDyve
Amplified ribosomal DNA restriction analysis (ARDRA) of
The polymerase chain reaction (PCR) and subsequent analysis of amplified rDNA using restriction endonucleases were employed in different studies topecies discrimination in achieve efficient inters medical [7] and food [8] mycology.
Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. 8 Pages. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene . Author. Denis Roy. Download with Google Download with Facebook or …
We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this
Evolutionary Relationships of Lentinus to the Polyporaceae: Evidence from Restriction Analysis of Enzymatically Amplified Ribosomal DNA David S. Hibbett; Rytas Vilgalys
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
RIBOSOMAL DNA RESTRICTION ENZYME ANALYSIS (ARDRA) N.H. Todorova1, G ARDRA (Amplified Ribosomal DNA Restriction enzyme Analysis) fingerprint method is chosen for assessment of dominant members of bacterial communities in root zone sediment of pristine Zostera meadows as well as in affected ones, as it allows the simultaneous analysis of multiple samples, …
Seven Euplotes spp. were investigated using amplified ribosomal DNA restriction analysis (RFLP, riboprinting). This involved the use of the polymerase chain reaction (PCR) to amplify a segment of the ciliate genome, followed by restriction digestion to yield restriction fragment length polymorphisms among the seven euplotids.
Olaf Schmidt and Ute Moreth, Identification of the Dry Rot Fungus, Serpula lacrymans, and the Wild Merulius, S. himantioides, by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Holzforschung, 53, 2, (1999).
Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1500 bp fragments of rDNA amplified by polymerase chain reaction did not discriminate the genomic variations among the eight strains of T. equigenitalis.
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
Practical identification of human originated Lactobacillus
Identification of Mycobacterium species by using amplified
Physicochemical Analysis After collection in the on-site laboratory, sludge samples were analyzed using conventional techniques as follows: sludge and air temperature, sludge volume index (SVI) [22],
Amplified ribosomal DNA restriction analysis (ARDRA) has an excellent potential for discrimination of organisms at the species level [6,7]. This technique is based on the amplification of the DNA sequence of a 16S rDNA region, followed by the digestion of PCR products with restriction enzymes [ 6 , 7 ].
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by …
Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified …
2 • Based on 16S ribosomal DNA gene (and possibly other genes) – 16S rDNA libraries and sequencing – Amplified ribosomal DNA restriction analysis (ARDRA)
Results demonstrate that the amplified ribosomal DNA restriction analysis (ARDRA) method allows G. vaginalis to be separated into at least 4 homogeneous genotypes, some of which showed a prevalent distribution in certain of the centers the strains were derived from.
Identification of Mycobacterium species by using amplified
Amplified ribosomal DNA restriction analysis of free
Amplified rDNA restriction analysis after separate digestion with five restriction enzymes, including AluI and MboI, of the 1500 bp fragments of rDNA amplified by polymerase chain reaction did not discriminate the genomic variations among the eight strains of T. equigenitalis.
Arthrospira (‘Spirulina’) strains from four continents are
Practical identification of human originated Lactobacillus species by amplified ribosomal DNA restriction analysis (ARDRA) for probiotic use
Identification of Mycobacterium Species by Using Amplified
ARDRA stands for “Amplified Ribosomal DNA Restriction Analysis”. Q: A: How to abbreviate “Amplified Ribosomal DNA Restriction Analysis”? “Amplified Ribosomal DNA Restriction Analysis” can be abbreviated as ARDRA. Q: A: What is the meaning of ARDRA abbreviation? The meaning of ARDRA abbreviation is “Amplified Ribosomal DNA Restriction Analysis”. Q: A: What …
Amplified ribosomal DNA restriction analysis in DeepDyve
Molecular Typing of Pasteurella pneumotropica Isolated
Use of amplified ribosomal DNA restriction analysis for
Results demonstrate that the amplified ribosomal DNA restriction analysis (ARDRA) method allows G. vaginalis to be separated into at least 4 homogeneous genotypes, some of which showed a prevalent distribution in certain of the centers the strains were derived from.
Amplified 16S Ribosomal DNA Restriction Analysis for
Original Research Amplified Ribosomal DNA Restriction
Comparison of Amplified Ribosomal DNA Restriction Analysis
Evolutionary Relationships of Lentinus to the Polyporaceae: Evidence from Restriction Analysis of Enzymatically Amplified Ribosomal DNA David S. Hibbett; Rytas Vilgalys
Amplified ribosomal DNA restriction analysis of free
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
Amplified ribosomal DNA restriction analysis (ARDRA) of
ABSTRACT. Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting.
Identification of Acinetobacter genomic species by
RIBOSOMAL DNA RESTRICTION ENZYME ANALYSIS (ARDRA) N.H. Todorova1, G ARDRA (Amplified Ribosomal DNA Restriction enzyme Analysis) fingerprint method is chosen for assessment of dominant members of bacterial communities in root zone sediment of pristine Zostera meadows as well as in affected ones, as it allows the simultaneous analysis of multiple samples, …
AMplified 16S Ribosomal DNA Restriction Analysis for
Identification of the ectomycorrhizal basidiomycete
An Application of Amplified Ribosomal DNA Restriction
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
Open Research Detection and identification of
Identification of the ectomycorrhizal basidiomycete
Amplified ribosomal DNA restriction analysis (ARDRA) of
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
Identification of Acinetobacter genomic species by
pdf. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) 10 Pages. Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda) Author. Igor Tiago. Download with
Identification of Mycobacterium by Amplified Ribosomal DNA
Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal …
Use of Amplified Ribosomal DNA Restriction Analysis for
Comparison of 16S amplified ribosomal DNA restriction analysis (ARDRA) patterns of fourteen reference Lactobacillus species The in silico patterns obtained from the almost complete 16S-rRNA analysis of 14 Lactobacillus species were compared with experimental patterns of 14 reference Lactobacillus species.
Amplified Ribosomal DNA Restriction Analysis
Microbiology Society Journals Characterization of
Amplified Ribosomal DNA Restriction Analysis Using Taq1
˜2016 152 115 Identifying the Origin of Forensic Soil Evidence Using Amplified Ribosomal DNA Restriction Analysis of its Bacterial Community Venus Naknim1, Wibhu Kutanan2 and …
Evaluation of amplified ribosomal DNA restriction analysis
Arthrospira (‘Spirulina’) strains from four continents are
Amplified ribosomal DNA restriction analysis (ARDRA) of
RIBOSOMAL DNA RESTRICTION ENZYME ANALYSIS (ARDRA) N.H. Todorova1, G ARDRA (Amplified Ribosomal DNA Restriction enzyme Analysis) fingerprint method is chosen for assessment of dominant members of bacterial communities in root zone sediment of pristine Zostera meadows as well as in affected ones, as it allows the simultaneous analysis of multiple samples, …
(PDF) Comparison of Amplified Ribosomal DNA Restriction
Comparison of the Value of Pulsed-field Gel
Seven Euplotes spp. were investigated using amplified ribosomal DNA restriction analysis (RFLP, riboprinting). This involved the use of the polymerase chain reaction (PCR) to amplify a segment of the ciliate genome, followed by restriction digestion to yield restriction fragment length polymorphisms among the seven euplotids.
Identification of Mycobacterium Species by Using Amplified
An Application of Amplified Ribosomal DNA Restriction
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
Application of amplified ribosomal DNA restriction
The phylogenetic relationships of the microsporidian species Microgemma caulleryi, Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA).
Original Research Amplified Ribosomal DNA Restriction
Use of amplified ribosomal DNA restriction analysis for identification of Ralstonia and Pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis.
Research Note Application of amplified ribosomal DNA
Arthrospira (‘Spirulina’) strains from four continents are
Amplified ribosomal DNA restriction analysis in DeepDyve
of these methods are based on PCR and restriction site analysis. Some of these methods are still not economic in use and Some of these methods are still not economic in use and require huge set-up cost.
Microbiology Society Journals Characterization of
Amplified 16S Ribosomal DNA Restriction Analysis for
Molecular characterization and amplified ribosomal DNA
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
Open Research Detection and identification of
An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus
Use of Amplified Ribosomal DNA Restriction Analysis for
Amplified ribosomal DNA restriction analysis as a routine
Evolutionary Relationships of Lentinus to the Polyporaceae
How to Cite this Article: Afaf I. Shehata, et al,. “Molecular Identification Of Probiotics Lactobacillus Strain isolates by: Amplified Ribosomal DNA Restriction Analysis (AR-
Identification of the ectomycorrhizal basidiomycete
PDF Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA
Open Research Detection and identification of
Amplified Ribosomal DNA Restriction Analysis Using Taq1
This corrects the article “Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.” on page 2063. Full text Get a printable copy (PDF file) of the complete article (200K), or click on a page image below to browse page by page.
Identification of Weissella species by the genus‐specific
Arthrospira (‘Spirulina’) strains from four continents are
Amplified Ribosomal DNA Restriction Analysis
2 • Based on 16S ribosomal DNA gene (and possibly other genes) – 16S rDNA libraries and sequencing – Amplified ribosomal DNA restriction analysis (ARDRA)
Identification of the ectomycorrhizal basidiomycete
Based on Amplified Ribosomal DNA Restriction Analysis (ARDRA) DIANA ELIZABETH WATURANGI ∗∗∗ , IVANA FRANCISCA, CINDY OKTAVIA SUSANTO Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia,
Identification of the ectomycorrhizal basidiomycete
It is important to determine the minimum number of restriction enzymes for examining the phylogenetic affiliation of the many isolates from environments using RFLP analysis of PCR amplified interspacer rDNA (17). Sneath and Sokal. Dendrogram obtained by unweighed pair group method using arithmetic averages (UPGMA. this strain was grouped with its original group I strains at 55% similarity. PCR
Arthrospira (‘Spirulina’) strains from four continents are
Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length polymorphism) to the gene encoding the small (16s) ribosomal …
Amplified ribosomal DNA restriction analysis for the
Amplified ribosomal DNA restriction analysis (ARDRA) For each isolate, 10 ll of PCR-amplified 16S rDNA was digested for 4 h at 37 C with 5 U of restriction endonuclease CfoI, HinfI, RsaI, DdeI, Sau3AI, AluI, HaeIII, and MspI (Bangalore GeNei, India) according to the manufacturer’s instructions and separated by electrophoresis on 2 % agarose gels in 19 Tris–acetate–EDTA buffer for 2 h at
Restriction analysis of the amplified ribosomal DNA
A molecular technique based on the restriction fragment length polymorphism of the 16S ribosomal genes amplified by a polymerase chain reaction (PCR), referred to as amplified 16S ribosomal DNA restriction analysis (ARDRA), was designed to identify 19 Avibacterium paragallinarum strains isolated from infraorbital sinus and nasal turbinate bone samples of broiler chickens, breeders, and laying
Arthrospira (‘Spirulina’) strains from four continents are
discrimination of the strains by amplified ribosomal DNA restriction analysis, and thus the internally transcribed spacer was selected as molecular taxonomic marker. The internally transcribed spacer sequences situated between the 16S and the 23 S rRNA were amplified by polymerase chain reaction and yielded amplicons of about 540 bp. Direct use of cells for polymerase chain reaction seemed to
Use of Amplified Ribosomal DNA Restriction Analysis for
Amplified ribosomal DNA restriction analysis (ARDRA) of
Olaf Schmidt and Ute Moreth, Identification of the Dry Rot Fungus, Serpula lacrymans, and the Wild Merulius, S. himantioides, by Amplified Ribosomal DNA Restriction Analysis (ARDRA), Holzforschung, 53, 2, (1999).
Use of amplified ribosomal DNA restriction analysis for
This corrects the article “Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.” on page 2063. Full text Get a printable copy (PDF file) of the complete article (200K), or click on a page image below to browse page by page.
Identification of Mycobacterium by Amplified Ribosomal DNA
Use of amplified ribosomal DNA restriction analysis for
Identification of Mycobacterium species by using amplified
Comparison of 16S amplified ribosomal DNA restriction analysis (ARDRA) patterns of fourteen reference Lactobacillus species The in silico patterns obtained from the almost complete 16S-rRNA analysis of 14 Lactobacillus species were compared with experimental patterns of 14 reference Lactobacillus species.
Arthrospira (‘Spirulina’) strains from four continents are
Amplified Ribosomal DNA Restriction Analysis
The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production
Amplified Ribosomal DNA Restriction Analysis Wikipedia
Research Note Application of amplified ribosomal DNA
We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA restriction analysis (ARDRA). The in vitro antagonistic activity of these endophytes against the phytopathogen Alternaria alternata was assayed by dual culture technique. Based on this
Evolutionary Relationships of Lentinus to the Polyporaceae
jurnal RFLP.pdf Gel Electrophoresis Agarose Gel
Open Research Detection and identification of
It is important to determine the minimum number of restriction enzymes for examining the phylogenetic affiliation of the many isolates from environments using RFLP analysis of PCR amplified interspacer rDNA (17). Sneath and Sokal. Dendrogram obtained by unweighed pair group method using arithmetic averages (UPGMA. this strain was grouped with its original group I strains at 55% similarity. PCR
Species separation among seven Euplotes spp. (Protozoa
jurnal RFLP.pdf Gel Electrophoresis Agarose Gel
enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species ofthe genus Mycobacterium were enzymatically amplified.
The Variability in the Fungal Ribosomal DNA (ITS1 ITS2
Amplified ribosomal DNA restriction analysis for the
Research Note Application of amplified ribosomal DNA
Physicochemical Analysis After collection in the on-site laboratory, sludge samples were analyzed using conventional techniques as follows: sludge and air temperature, sludge volume index (SVI) [22],
Use of amplified ribosomal DNA restriction analysis for
Amplified Ribosomal DNA Restriction Analysis Using Taq1
Read “Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae : a contribution to the study of these free-living nitrogen-fixing bacteria, Journal Of Microbiological Methods” on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Comparison of the Value of Pulsed-field Gel
Identification of Weissella species by the genus‐specific
In this context, the potential of amplified ribosomal DNA restriction analysis (ARDRA) for the characterization and differentiation of Azotobacteraceae was evaluated, and the more appropriate tool for the elaboration of data was assessed.
jurnal RFLP.pdf Gel Electrophoresis Agarose Gel
(PDF) Comparison of Amplified Ribosomal DNA Restriction
Molecular characterization by amplified ribosomal DNA
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
Identification of Acinetobacter genomic species by
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
Molecular differentiation of Bifidobacterium species with
Molecular methods to assess microbial diversity life.umd.edu
Amplified Ribosomal DNA Restriction Analysis. Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.
Microbiology Society Journals Characterization of
Physicochemical Analysis After collection in the on-site laboratory, sludge samples were analyzed using conventional techniques as follows: sludge and air temperature, sludge volume index (SVI) [22],
AMplified 16S Ribosomal DNA Restriction Analysis for
Restriction analysis of the amplified ribosomal DNA
Application of amplified ribosomal DNA restriction
Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of
Amplified ribosomal DNA restriction analysis of free
Amplified Ribosomal DNA Restriction Analysis Using Taq1
PDF Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA
Amplified ribosomal DNA restriction analysis as a routine
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
Identification of Mycobacterium by Amplified Ribosomal DNA
Comparison of Amplified Ribosomal DNA Restriction Analysis
Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia Kong, B.H. and Hanifah, Y.A. and Yusof, M.Y. and Thong, Kwai Lin (2011) Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia.
Open Research Detection and identification of
Identification of Weissella species by the genus‐specific
Genotypic differentiation of Gardnerella vaginalis by
2 • Based on 16S ribosomal DNA gene (and possibly other genes) – 16S rDNA libraries and sequencing – Amplified ribosomal DNA restriction analysis (ARDRA)
Identification of Mycobacterium species by using amplified
ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by …
Amplified ribosomal DNA restriction analysis (ARDRA) of
The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species.
Molecular Identification Of Probiotics Lactobacillus
Molecular characterization and amplified ribosomal DNA
How to Cite this Article: Afaf I. Shehata, et al,. “Molecular Identification Of Probiotics Lactobacillus Strain isolates by: Amplified Ribosomal DNA Restriction Analysis (AR-
Evolutionary Relationships of Lentinus to the Polyporaceae
Comparison of 16S amplified ribosomal DNA restriction analysis (ARDRA) patterns of fourteen reference Lactobacillus species The in silico patterns obtained from the almost complete 16S-rRNA analysis of 14 Lactobacillus species were compared with experimental patterns of 14 reference Lactobacillus species.
Amplified Ribosomal DNA Restriction Analysis as a Tool to
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
Identification of Mycobacterium species by using amplified
Results demonstrate that the amplified ribosomal DNA restriction analysis (ARDRA) method allows G. vaginalis to be separated into at least 4 homogeneous genotypes, some of which showed a prevalent distribution in certain of the centers the strains were derived from.
The Variability in the Fungal Ribosomal DNA (ITS1 ITS2
Amplified ribosomal DNA restriction analysis of free
Amplified ribosomal DNA restriction analysis (ARDRA) proved to be useful for the classification of bacterial strains at different taxonomic levels and to analyze the genetic variability between bacterial isolates depending on selection of conserved or variable regions in the ribosomal …
Amplified Ribosomal DNA Restriction Analysis as a Tool to
Use of Amplified Ribosomal DNA Restriction Analysis for
Genetic Diversity of Methylotrophic Bacteria from Human
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
Amplified Ribosomal DNA Restriction Analysis
Amplified ribosomal DNA restriction analysis (ARDRA) of new isolated strains of bifidobacteria from newborn babies microbiota. microbial flora, microorganisms, neonates, pH, probiotics, ribosomal DNA, strains, tolerance Identifier(s) : bacterium, gall, gut flora
Practical identification of human originated Lactobacillus
This corrects the article “Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.” on page 2063. Full text Get a printable copy (PDF file) of the complete article (200K), or click on a page image below to browse page by page.
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
Amplified ribosomal DNA restriction analysis in DeepDyve
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
Molecular characterization by amplified ribosomal DNA
An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus
Microbiology Society Journals Characterization of
Identification of Mycobacterium species by using amplified
Identification of Acinetobacter genomic species by
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
Molecular Typing of Pasteurella pneumotropica Isolated
Evaluation of amplified ribosomal DNA restriction analysis
The phylogenetic relationships of the microsporidian species Microgemma caulleryi, Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA).
Amplified Ribosomal DNA Restriction Analysis Using Taq1
Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of
RFLP analysis of PCR-amplified small subunit ribosomal DNA
Molecular differentiation of Bifidobacterium species with
Comparison of Amplified Ribosomal DNA Restriction Analysis
PDF Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA
Identification of Mycobacterium species by using amplified
Identification of Mycobacterium Species by Using Amplified
Amplified 16S Ribosomal DNA Restriction Analysis for
An Application of Amplified Ribosomal DNA Restriction Analysis in the Changes of Microbial Community Structure of Industrial Waste Water Treatment by M. Shah International Journal of Environmental Bioremediation & Biodegradation .
Molecular characterization and amplified ribosomal DNA
RFLP analysis of PCR-amplified small subunit ribosomal DNA
Identification of Mycobacterium Species by Using Amplified
Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene. 8 Pages. Molecular differentiation of Bifidobacterium species with amplified ribosomal DNA restriction analysis and alignment of short regions of the ldh gene . Author. Denis Roy. Download with Google Download with Facebook or …
Identifying the Origin of Forensic Soil Evidence Using
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
Molecular Identification Of Probiotics Lactobacillus
Comparison of the Value of Pulsed-field Gel
Application of amplified ribosomal DNA restriction
Amplified Ribosomal DNA Restriction Analysis. Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria.
Research Note Application of amplified ribosomal DNA
Amplified ribosomal DNA restriction analysis in DeepDyve
2 • Based on 16S ribosomal DNA gene (and possibly other genes) – 16S rDNA libraries and sequencing – Amplified ribosomal DNA restriction analysis (ARDRA)
Molecular Identification Of Probiotics Lactobacillus
Arthrospira (‘Spirulina’) strains from four continents are
Read “Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae : a contribution to the study of these free-living nitrogen-fixing bacteria, Journal Of Microbiological Methods” on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
AMplified 16S Ribosomal DNA Restriction Analysis for
Molecular characterization and amplified ribosomal DNA
Comparison of the Value of Pulsed-field Gel
Amplified ribosomal DNA restriction analysis (ARDRA) For each isolate, 10 ll of PCR-amplified 16S rDNA was digested for 4 h at 37 C with 5 U of restriction endonuclease CfoI, HinfI, RsaI, DdeI, Sau3AI, AluI, HaeIII, and MspI (Bangalore GeNei, India) according to the manufacturer’s instructions and separated by electrophoresis on 2 % agarose gels in 19 Tris–acetate–EDTA buffer for 2 h at
RFLP analysis of PCR-amplified small subunit ribosomal DNA
Identification of Mycobacterium Species by Using Amplified
Molecular biological methods based on genus-specific PCR, species-specific PCR, and amplified ribosomal DNA restriction analysis (ARDRA) of two PCR amplicons (523 and 914 bp) using six restriction enzymes were used to differentiate among species of Bifidobacterium.
Use of amplified ribosomal DNA restriction analysis for
Amplified ribosomal DNA restriction analysis (ARDRA) For each isolate, 10 ll of PCR-amplified 16S rDNA was digested for 4 h at 37 C with 5 U of restriction endonuclease CfoI, HinfI, RsaI, DdeI, Sau3AI, AluI, HaeIII, and MspI (Bangalore GeNei, India) according to the manufacturer’s instructions and separated by electrophoresis on 2 % agarose gels in 19 Tris–acetate–EDTA buffer for 2 h at
An Application of Amplified Ribosomal DNA Restriction
Amplified ribosomal DNA restriction analysis (ARDRA) has an excellent potential for discrimination of organisms at the species level [6,7]. This technique is based on the amplification of the DNA sequence of a 16S rDNA region, followed by the digestion of PCR products with restriction enzymes [ 6 , 7 ].
Molecular characterization and amplified ribosomal DNA
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
It is important to determine the minimum number of restriction enzymes for examining the phylogenetic affiliation of the many isolates from environments using RFLP analysis of PCR amplified interspacer rDNA (17). Sneath and Sokal. Dendrogram obtained by unweighed pair group method using arithmetic averages (UPGMA. this strain was grouped with its original group I strains at 55% similarity. PCR
Application of amplified ribosomal DNA restriction
(PDF) Comparison of Amplified Ribosomal DNA Restriction
Use of Amplified Ribosomal DNA Restriction Analysis for
˜2016 152 115 Identifying the Origin of Forensic Soil Evidence Using Amplified Ribosomal DNA Restriction Analysis of its Bacterial Community Venus Naknim1, Wibhu Kutanan2 and …
Practical identification of human originated Lactobacillus
Identification of Mycobacterium Species by Using Amplified
fig11379: Amplified Ribosomal DNA Restriction Analysis Using Taq1 (TCGA) Mentions: Figure 2 demonstrates that all the isolates of 1114 bp were specific to bacilli. Taq1 digested the PCR product identically and produced two amplicons with approximate sizes of 420 and 690 bp (Figure 3).
Genotypic differentiation of Gardnerella vaginalis by
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
Application of amplified ribosomal DNA restriction
Molecular differentiation of Bifidobacterium species with
Amplified ribosomal DNA restriction analysis of free
ARDRA (Amplified ribosomal DNA restriction analysis) is a simple method based on restriction endonuclease digestion of the amplified bacterial 16S rDNA. In this study we have evaluated the suitability of this method to detect differences in activated sludge bacterial communities fed on domestic or industrial wastewater, and subject to different
Use of amplified ribosomal DNA restriction analysis for
Amplified ribosomal DNA restriction analysis as a routine
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
ARDRA Amplified Ribosomal DNA Restriction Analysis in
Restriction analysis of the amplified ribosomal DNA
The phylogenetic relationships of the microsporidian species Microgemma caulleryi, Pleistophora finisterrensis and Tetramicra brevifilum were investigated on the basis of restriction fragment length polymorphism (RFLP) analysis of PCR-amplified small-subunit rDNA (SSUrDNA).
Species separation among seven Euplotes spp. (Protozoa
Amplified 16S Ribosomal DNA Restriction Analysis for
An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus
Molecular Identification Of Probiotics Lactobacillus
An Application of Amplified Ribosomal DNA Restriction
Molecular Typing of Pasteurella pneumotropica Isolated
Use of amplified ribosomal DNA restriction analysis for identification of Ralstonia and Pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis.
Amplified 16S Ribosomal DNA Restriction Analysis for
Molecular Typing of Pasteurella pneumotropica Isolated
Amplified ribosomal DNA restriction analysis as a routine
Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of
Amplified Ribosomal DNA Restriction Analysis
Original Research Amplified Ribosomal DNA Restriction
Application of amplified ribosomal DNA restriction
fig11379: Amplified Ribosomal DNA Restriction Analysis Using Taq1 (TCGA) Mentions: Figure 2 demonstrates that all the isolates of 1114 bp were specific to bacilli. Taq1 digested the PCR product identically and produced two amplicons with approximate sizes of 420 and 690 bp (Figure 3).
Comparison of the Value of Pulsed-field Gel
Application of amplified ribosomal DNA restriction
A total of 53 field and reference strains, including the type strains of the seven named species (nomenspecies) and belonging to the 18 described genomic species (DNA groups) of the genus Acinetobacter, were studied by amplified ribosomal DNA restriction analysis (ARDRA).
Molecular characterization by amplified ribosomal DNA
AMplified 16S Ribosomal DNA Restriction Analysis for
An Application of Amplified Ribosomal DNA Restriction Analysis in the Changes of Microbial Community Structure of Industrial Waste Water Treatment by M. Shah International Journal of Environmental Bioremediation & Biodegradation .
ARDRA Amplified Ribosomal DNA Restriction Analysis in
Identification of Mycobacterium Species by Using Amplified
fig11379: Amplified Ribosomal DNA Restriction Analysis Using Taq1 (TCGA) Mentions: Figure 2 demonstrates that all the isolates of 1114 bp were specific to bacilli. Taq1 digested the PCR product identically and produced two amplicons with approximate sizes of 420 and 690 bp (Figure 3).
Amplified ribosomal DNA restriction analysis of free
Amplified ribosomal DNA restriction analysis (ARDRA) proved to be useful for the classification of bacterial strains at different taxonomic levels and to analyze the genetic variability between bacterial isolates depending on selection of conserved or variable regions in the ribosomal …
Identification of Weissella species by the genus‐specific
Amplified ribosomal DNA restriction analysis of free
An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus
Identification of the ectomycorrhizal basidiomycete
Microbiology Society Journals Characterization of
Application of amplified ribosomal DNA restriction
electrophoresis of fragments of ribosomal RNA gene (rDNA), generated by PCR, and sub- sequent digestions with restriction enzymes (ARDRA – amplified ribosomal DNA restriction analysis).
ARDRA Amplified Ribosomal DNA Restriction Analysis in
Identification of the ectomycorrhizal basidiomycete
Identification of Acinetobacter genomic species by
Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia Kong, B.H. and Hanifah, Y.A. and Yusof, M.Y. and Thong, Kwai Lin (2011) Application of amplified ribosomal DNA restriction analysis in identification of Acinetobacter baumannii from a tertiary teaching hospital, Malaysia.
Evaluation of amplified ribosomal DNA restriction analysis
Practical identification of human originated Lactobacillus
The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species.
(PDF) Comparison of Amplified Ribosomal DNA Restriction
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
Amplified ribosomal DNA restriction analysis in DeepDyve
In this study, another molecular biology technique, the amplified ribosomal DNA restriction analysis (ARDRA), is applied to activated sludge samples. Even faster than hybridization and probing, ARDRA has been used in the analysis of mixed bacterial populations from different environments [ 47 , 48 , 49 ] .
Genotypic differentiation of Gardnerella vaginalis by
Use of amplified ribosomal DNA restriction analysis for
In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
The Variability in the Fungal Ribosomal DNA (ITS1 ITS2
RESTRICTION ANALYSIS OF THE RIBOSOMAL DNA INTERNAL
Original Research Amplified Ribosomal DNA Restriction
564 (Yamamoto & Harayama, 1996) and recA-RFLP (Jawad et al., 1998). Among these, amplified ribosomal DNA restriction analysis (ARDRA) was reported to be the useful
Amplified Ribosomal DNA Restriction Analysis as a Tool to
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
electrophoresis of fragments of ribosomal RNA gene (rDNA), generated by PCR, and sub- sequent digestions with restriction enzymes (ARDRA – amplified ribosomal DNA restriction analysis).
Amplified ribosomal DNA restriction analysis (ARDRA) of
MICROBIAL DIVERSITY IN ZOSTERA SP. BEDS IN SOUTH-
Microbiology Society Journals Characterization of
Amplified ribosomal DNA restriction analysis (ARDRA) of new isolated strains of bifidobacteria from newborn babies microbiota. microbial flora, microorganisms, neonates, pH, probiotics, ribosomal DNA, strains, tolerance Identifier(s) : bacterium, gall, gut flora
Molecular methods to assess microbial diversity life.umd.edu
Molecular characterization and amplified ribosomal DNA
Genetic Diversity of Methylotrophic Bacteria from Human
Abstract. The Weissella species have been increasingly isolated from many food and meat samples. For rapid and systematic identification of Weissella species, an amplified ribosomal DNA restriction analysis (ARDRA) has been developed.
Molecular characterization and amplified ribosomal DNA
(PDF) Comparison of Amplified Ribosomal DNA Restriction
This study showed that a polymerase chain reaction named ARDRA (Amplified 16S Ribosomal DNA Restriction Analysis) method could prove to be an invaluable insight for molecular identification of the A. paragallinarum, a microorganism implicated in respiratory diseases in commercial birds.
Molecular differentiation of Bifidobacterium species with
Original Research Amplified Ribosomal DNA Restriction
A rapid procedure for the identification of cultured Mycobacterium isolates, based on the combination of enzymatic amplification and restriction analysis, is described. The 16S rRNA genes (rDNA) of 99 strains belonging to 18 different species of the genus Mycobacterium were enzymatically amplified
Identification of the ectomycorrhizal basidiomycete
Restriction fragment length polymorphism (RFLP) is a technique invented in 1984 by the English scientist Alec Jeffreys during research into hereditary diseases. It is used for the analysis of
Restriction analysis of the amplified ribosomal DNA
Comparison of Amplified Ribosomal DNA Restriction Analysis
Identification of Mycobacterium by Amplified Ribosomal DNA
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